Together with the reverse transcription (RT) reaction, the PCR method has been widely applied to the quantitative measurement of DNA and RNA messages. parvum using the Anyplex™ STI-7 detection kit in urine may indicate a high probability of infection rather than colonization, especially in women and young patients.Polymerase chain reaction (PCR) is a very powerful tool for qualitative evaluation of nucleic acids due to its high efficiency and convenience. The significant difference between the symptomatic and asymptomatic group in the highest positivity group for U. urealyticum in all groups by age, gender, and strength of positivity. parvum with low strength of positivity and for U. No significant differences were observed between the prevalence in symptomatic and asymptomatic patients of U. This finding was observed especially in women and in the young group (15-35 years of age). parvum was significantly more prevalent in symptomatic compared to asymptomatic patients. A total of 250 symptomatic and 250 asymptomatic controls were included.Ī strong positive result for U. urealyticum using a semi-quantitative multiplex PCR technique for sexually transmitted diseases (Anyplex™ STI-7 Detection Kit, Seegene, South Korea). The aim of the study was to evaluate the efficacy of a quantitative polymerase chain reaction (qPCR) method for the discrimination between infection and colonization by measuring prevalence of Usp in asymptomatic versus symptomatic patients. Because they can also just colonize the genital tract of healthy people, their pathogenic role can be difficult to prove. Usp may be commensals in the genital tract but may also be contributors to a number of pathological conditions of the genital tract. Ureaplasma species (Usp) are the most prevalent genital Mycoplasma isolated from the urogenital tract of both men and women.
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